PCR-Oligonucleotide Ligation Assay from Dried Blood Spots
نویسندگان
چکیده
منابع مشابه
PCR-oligonucleotide ligation assay from dried blood spots.
process, and they work together to effect the amniochorionic system that is the main source for production not only of IL-8 (13 ) but also of various prostanoids (14, 15). Therefore, the highly variable concentrations of IL-8 in the CVS may be attributable to the extent of and/or the stages in which these factors contribute to cervical-decidualamniochorionic activation. However, the precise mec...
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The multi-region hybridization assay (MHAbce) for genotyping HIV-1 subtypes B, C and circulating recombinant form (CRF01_AE) was evaluated on paired plasma and dried blood spots (DBS) collected from 68 HIV-1 infected individuals in Thailand. CRF01_AE was the predominant subtype identified using plasma samples (51/62) and DBS (24/27). There was no discordance in subtype designations between plas...
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We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
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T h e collection of dried blood spots on filter paper blotters is a major innovation and has facilitated the development and dissemination of newborn screening programs principally because of the ease of sample collection, handling, and shipment to centrally located laboratories and the stability of the analytes in these specimens. (1,2) These samples, which are also known as "Guthrie spots," a...
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BACKGROUND Assays have been developed for cross-sectional HIV incidence estimation using plasma samples. Large scale surveillance programs are planned using dried blood spot (DBS) specimens for incidence assessment. However, limited information exists on the performance of HIV cross-sectional incidence assays using DBS. METHODS The assays evaluated were: Maxim HIV-1 Limiting Antigen Avidity E...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1999
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/45.11.2022